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mtb h37rv reference strain (ATCC)

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ATCC mtb h37rv reference strain
Schematic of the experimental workflow for method validation using <t>H37Rv-spiked</t> dust samples

Mtb H37rv Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtb+reference+strain+h37rv/Mycobacterium+tuberculosis%3B+subsp%2E+tuberculosis/pmc12870882-49-1-10
Average 98 stars, based on 2908 article reviews

mtb h37rv reference strain - by Bioz Stars, 2026-09

98/100 stars

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1) Product Images from "Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust"

Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

Journal: BMC Infectious Diseases

doi: 10.1186/s12879-025-12332-0

Figure Legend Snippet: Schematic of the experimental workflow for method validation using H37Rv-spiked dust samples

Techniques Used: Biomarker Discovery

$Determination of optimal Percoll density gradient for H37Rv separation from dust. ( A , D) Photographs of discontinuous ( A ) 5-layer and ( D ) 3-layer Percoll density gradients. Methylene blue was added to select layers during gradient preparation for visual clarity. The distinct interphases after centrifugation are formed by intrinsic density differences. Pure H37Rv (acid-fast stained, red bacilli) and unprocessed dust particles were layered. The pink background in the MTB sample tubes originates from the suspension medium of the stained bacilli; the actual localization of MTB is indicated by the turbid, cloudy bands at the density interfaces. ( B , E ) Quantitative comparison of H37Rv DNA recovery efficiency across gradient fractions, as determined by qPCR. Data are presented as mean ± SD ( n = 3). Statistical significance was analyzed by one-way ANOVA for multi-group comparisons and unpaired t-test for pairwise comparisons (*** p < 0.001, **** p < 0.0001). ( C ) Representative microscopic fields (1000× magnification) of acid-fast stained bacilli recovered from each layer of the 5-layer gradient (scale bar: 100 μm)$
Figure Legend Snippet: Determination of optimal Percoll density gradient for H37Rv separation from dust. ( A , D) Photographs of discontinuous ( A ) 5-layer and ( D ) 3-layer Percoll density gradients. Methylene blue was added to select layers during gradient preparation for visual clarity. The distinct interphases after centrifugation are formed by intrinsic density differences. Pure H37Rv (acid-fast stained, red bacilli) and unprocessed dust particles were layered. The pink background in the MTB sample tubes originates from the suspension medium of the stained bacilli; the actual localization of MTB is indicated by the turbid, cloudy bands at the density interfaces. ( B , E ) Quantitative comparison of H37Rv DNA recovery efficiency across gradient fractions, as determined by qPCR. Data are presented as mean ± SD ( n = 3). Statistical significance was analyzed by one-way ANOVA for multi-group comparisons and unpaired t-test for pairwise comparisons (*** p < 0.001, **** p < 0.0001). ( C ) Representative microscopic fields (1000× magnification) of acid-fast stained bacilli recovered from each layer of the 5-layer gradient (scale bar: 100 μm)

Techniques Used: Centrifugation, Staining, Suspension, Comparison

Viability assessment of H37Rv recovered via Percoll separation. ( A-E ) Macroscopic appearance of H37Rv colonies on LJ medium after 4 weeks of incubation. Left panels: Recovery standard controls (direct inoculation of pure H37Rv suspensions without dust). Right panels: Colonies recovered via Percoll separation from H37Rv-spiked dust samples. Initial spiking concentrations: ( A ) 10⁰, ( B ) 10¹, ( C ) 10², ( D ) 10³, ( E ) 10⁴ CFU/mL. ( F ) Quantitative comparison of viable MTB concentrations (CFU/mL) between Percoll-separated samples (from dust matrix) and direct inoculation controls (pure H37Rv). Colony counts were converted to CFU/mL for analysis. Bars represent mean ± SD ( n = 3). Statistical significance was determined by a paired t-test (ns, not significant; ** p < 0.01)

Figure Legend Snippet: Viability assessment of H37Rv recovered via Percoll separation. ( A-E ) Macroscopic appearance of H37Rv colonies on LJ medium after 4 weeks of incubation. Left panels: Recovery standard controls (direct inoculation of pure H37Rv suspensions without dust). Right panels: Colonies recovered via Percoll separation from H37Rv-spiked dust samples. Initial spiking concentrations: ( A ) 10⁰, ( B ) 10¹, ( C ) 10², ( D ) 10³, ( E ) 10⁴ CFU/mL. ( F ) Quantitative comparison of viable MTB concentrations (CFU/mL) between Percoll-separated samples (from dust matrix) and direct inoculation controls (pure H37Rv). Colony counts were converted to CFU/mL for analysis. Bars represent mean ± SD ( n = 3). Statistical significance was determined by a paired t-test (ns, not significant; ** p < 0.01)

Techniques Used: Incubation, Comparison

Detection of MTB in air-conditioning dust from TB hospital wards using qPCR. ( A–D ) Representative qPCR amplification curves for MTB DNA detected in 25 clinical dust samples. Dashed lines: samples processed by Percoll separation. Solid lines: samples processed by direct DNA extraction. ( E ) qPCR amplification profiles for positive controls (H37Rv genomic DNA, red lines) and negative controls (no-template, green lines). ( F ) Examples of abnormal amplification curves obtained from the direct extraction method. ( G ) Violin plots comparing MTB DNA copy numbers between Percoll-based separation and direct extraction methods. Horizontal lines within violins represent the median and interquartile range. Statistical significance was determined by the Mann-Whitney U test (**** p < 0.0001)

Figure Legend Snippet: Detection of MTB in air-conditioning dust from TB hospital wards using qPCR. ( A–D ) Representative qPCR amplification curves for MTB DNA detected in 25 clinical dust samples. Dashed lines: samples processed by Percoll separation. Solid lines: samples processed by direct DNA extraction. ( E ) qPCR amplification profiles for positive controls (H37Rv genomic DNA, red lines) and negative controls (no-template, green lines). ( F ) Examples of abnormal amplification curves obtained from the direct extraction method. ( G ) Violin plots comparing MTB DNA copy numbers between Percoll-based separation and direct extraction methods. Horizontal lines within violins represent the median and interquartile range. Statistical significance was determined by the Mann-Whitney U test (**** p < 0.0001)

Techniques Used: Amplification, DNA Extraction, Extraction, MANN-WHITNEY

Cell Culture:

Article Title: MiR-25 blunts autophagy and promotes the survival of Mycobacterium tuberculosis by regulating NPC1.
Article Snippet: Bafilomycin A1 (Baf-A1; Selleck, S1413), DAPI (Beyotime, C1005). .. Cell culture and bacterial strains The Mtb reference strain H37Rv (ATCC 27294) and BCG Tokyo strain (ATCC 35737) were cultured in Middlebrook 7H9 medium (Becton Dickinson, Difco, 271310) supplemented with 10% oleic acid, albumin, dextrose (OADC) (Becton Dickinson, Difco, 212352), 0.5% (v/v) glycerol (Sigma Aldrich, G5516), and 0.05% (v/v) Tween 80 (Sigma Aldrich, P8074). ..

Article Title: Antitubercular, Cytotoxicity, and Computational Target Validation of Dihydroquinazolinone Derivatives
Article Snippet: .. The MTB reference strain H37Rv (American Type Culture Collection (ATCC) 25177) and MDR-TB were cultured for a total of 3 weeks in Middlebrook 7H11 medium [ ] and were then supplemented with Oleic Albumin Dextrose Catalase (OADC) (0.005% v / v oleic acid, 0.2% w / v glucose, 0.085% w / v NaCl, 0.02% v / v catalase, and 0.5% 171 w / v bovine serum albumin (BSA)). ..

Article Title: MiR-25 blunts autophagy and promotes the survival of Mycobacterium tuberculosis by regulating NPC1
Article Snippet: Bafilomycin A1 (Baf-A1; Selleck, S1413), DAPI (Beyotime, C1005). .. The Mtb reference strain H37Rv (ATCC 27294) and BCG Tokyo strain (ATCC 35737) were cultured in Middlebrook 7H9 medium (Becton Dickinson, Difco, 271310) supplemented with 10% oleic acid, albumin, dextrose (OADC) (Becton Dickinson, Difco, 212352), 0.5% (v/v) glycerol (Sigma Aldrich, G5516), and 0.05% (v/v) Tween 80 (Sigma Aldrich, P8074). ..

Infection:

Article Title: Profiling the immune response to Mycobacterium tuberculosis Beijing family infection: a perspective from the transcriptome
Article Snippet: .. Cells were infected with 100 μl of each strain (three wells per strain and a control of cells without infection), and as a control we used Mtb reference strain H37Rv (ATCC 25,618). ..

Control:

Isolation:

Article Title: Lefamulin harbors promising anti-tuberculosis activity against multidrug-resistant Mycobacterium tuberculosis isolates
Article Snippet: .. Mtb reference strain H37Rv (ATCC 27294) and clinical isolated strains, including sensitive strains and MDR strains of Mtb, were stored in the Bio-bank in Beijing Chest Hospital. ..

Article Title: Ultrafast, One-Step, Label-Based Biosensor Diagnosis Platform for the Detection of Mycobacterium tuberculosis in Clinical Applications.
Article Snippet: Tuberculosis (TB) is a chronic infectious disease caused by the etiological agent Mycobacterium tuberculosis (MTB).. Because the majority of TB patients come from poor economic backgrounds, the development of a simple, specific, low-cost, and highly sensitive detection method for the pathogen is extremely important for the prevention and treatment of this disease.. In the current study, an efficient detection method for visual, rapid, and highly sensitive detection of MTB utilizing multiplex loop-mediated isothermal amplification combined with a label-based lateral flow immunoassay biosensor (mLAMP-LFIA) was developed.

Gentle:

Article Title: Effect of mycobacterial proteins that target mitochondria on the alveolar macrophages activation during Mycobacterium tuberculosis infection.
Article Snippet: Purpose of the study: During the early and progressive (late) stages of murine experimental pulmonary tuberculosis, the differential activation of macrophages contributes to disease development by controlling bacterial growth and immune regulation.. Mycobacterial proteins P27 and PE_PGRS33 can target the mitochondria of macrophages.. This study aims to evaluate the effect of both proteins on macrophage activation during mycobacterial infection.

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Journal: BMC Infectious Diseases

Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

doi: 10.1186/s12879-025-12332-0

Figure Lengend Snippet: Schematic of the experimental workflow for method validation using H37Rv-spiked dust samples

Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Biomarker Discovery

Journal: BMC Infectious Diseases

Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

doi: 10.1186/s12879-025-12332-0

Figure Lengend Snippet: Determination of optimal Percoll density gradient for H37Rv separation from dust. ( A , D) Photographs of discontinuous ( A ) 5-layer and ( D ) 3-layer Percoll density gradients. Methylene blue was added to select layers during gradient preparation for visual clarity. The distinct interphases after centrifugation are formed by intrinsic density differences. Pure H37Rv (acid-fast stained, red bacilli) and unprocessed dust particles were layered. The pink background in the MTB sample tubes originates from the suspension medium of the stained bacilli; the actual localization of MTB is indicated by the turbid, cloudy bands at the density interfaces. ( B , E ) Quantitative comparison of H37Rv DNA recovery efficiency across gradient fractions, as determined by qPCR. Data are presented as mean ± SD ( n = 3). Statistical significance was analyzed by one-way ANOVA for multi-group comparisons and unpaired t-test for pairwise comparisons (*** p < 0.001, **** p < 0.0001). ( C ) Representative microscopic fields (1000× magnification) of acid-fast stained bacilli recovered from each layer of the 5-layer gradient (scale bar: 100 μm)

Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Centrifugation, Staining, Suspension, Comparison

Journal: BMC Infectious Diseases

Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

doi: 10.1186/s12879-025-12332-0

Figure Lengend Snippet: Viability assessment of H37Rv recovered via Percoll separation. ( A-E ) Macroscopic appearance of H37Rv colonies on LJ medium after 4 weeks of incubation. Left panels: Recovery standard controls (direct inoculation of pure H37Rv suspensions without dust). Right panels: Colonies recovered via Percoll separation from H37Rv-spiked dust samples. Initial spiking concentrations: ( A ) 10⁰, ( B ) 10¹, ( C ) 10², ( D ) 10³, ( E ) 10⁴ CFU/mL. ( F ) Quantitative comparison of viable MTB concentrations (CFU/mL) between Percoll-separated samples (from dust matrix) and direct inoculation controls (pure H37Rv). Colony counts were converted to CFU/mL for analysis. Bars represent mean ± SD ( n = 3). Statistical significance was determined by a paired t-test (ns, not significant; ** p < 0.01)

Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Incubation, Comparison

Journal: BMC Infectious Diseases

Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

doi: 10.1186/s12879-025-12332-0

Figure Lengend Snippet: Detection of MTB in air-conditioning dust from TB hospital wards using qPCR. ( A–D ) Representative qPCR amplification curves for MTB DNA detected in 25 clinical dust samples. Dashed lines: samples processed by Percoll separation. Solid lines: samples processed by direct DNA extraction. ( E ) qPCR amplification profiles for positive controls (H37Rv genomic DNA, red lines) and negative controls (no-template, green lines). ( F ) Examples of abnormal amplification curves obtained from the direct extraction method. ( G ) Violin plots comparing MTB DNA copy numbers between Percoll-based separation and direct extraction methods. Horizontal lines within violins represent the median and interquartile range. Statistical significance was determined by the Mann-Whitney U test (**** p < 0.0001)

Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Amplification, DNA Extraction, Extraction, MANN-WHITNEY

The bacteriostatic activity of LEF against M. tuberculosis . The MBC of LEF against M. tuberculosis: ( A ) the PI and DAPI fluorescence values ( B ) and bacterial mortality rate ( C ) of H37Rv after co-incubation with 1/8 × MIC, 1/4 × MIC, 1 × MIC, 4 × MIC, 8 × MIC, and 64 × MIC of LEF for 7 days are shown. All data are presented as the means ± SD ( n = 3). **** P < 0.0001.

Journal: Microbiology Spectrum

Article Title: Lefamulin harbors promising anti-tuberculosis activity against multidrug-resistant Mycobacterium tuberculosis isolates

doi: 10.1128/spectrum.02250-25

Figure Lengend Snippet: The bacteriostatic activity of LEF against M. tuberculosis . The MBC of LEF against M. tuberculosis: ( A ) the PI and DAPI fluorescence values ( B ) and bacterial mortality rate ( C ) of H37Rv after co-incubation with 1/8 × MIC, 1/4 × MIC, 1 × MIC, 4 × MIC, 8 × MIC, and 64 × MIC of LEF for 7 days are shown. All data are presented as the means ± SD ( n = 3). **** P < 0.0001.

Article Snippet: Mtb reference strain H37Rv (ATCC 27294) and clinical isolated strains, including sensitive strains and MDR strains of Mtb, were stored in the Bio-bank in Beijing Chest Hospital.

Techniques: Activity Assay, Fluorescence, Incubation

Time-kill curves of LEF ( A ), BDQ ( B ), and INH ( C ) against M. tuberculosis H37Rv. Antibiotic concentrations are presented as different symbols. The DMSO was used as a NC.

Journal: Microbiology Spectrum

Article Title: Lefamulin harbors promising anti-tuberculosis activity against multidrug-resistant Mycobacterium tuberculosis isolates

doi: 10.1128/spectrum.02250-25

Figure Lengend Snippet: Time-kill curves of LEF ( A ), BDQ ( B ), and INH ( C ) against M. tuberculosis H37Rv. Antibiotic concentrations are presented as different symbols. The DMSO was used as a NC.

Article Snippet: Mtb reference strain H37Rv (ATCC 27294) and clinical isolated strains, including sensitive strains and MDR strains of Mtb, were stored in the Bio-bank in Beijing Chest Hospital.

Techniques:

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mtb h37rv reference strain (ATCC)

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